Journal of Traditional Chinese Medicine ›› 2021, Vol. 41 ›› Issue (4): 600-607.DOI: 10.19852/j.cnki.jtcm.2021.04.009

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Salvia miltiorrhiza-asarum ointment combined with Chinese medical massage alleviates symptoms of osteoarthritis in a rat model through the Notch1/matrix metalloproteinase-13 signaling pathway

LAI Qingzhong, ZHANG Xianjie, CHEN Meilan, HAN Zhicun   

  1. Department of Chinese Medical Massage,Zhejiang Integrated Traditional and Western Medicine Hospital;Department of Orthopedics,Jinhua First People's Hospital of Wucheng district;Department of Acupuncture,Tongde Hospital of Zhejiang Province;
  • Online:2021-08-06 Published:2021-01-20
  • Supported by:
    Supported by the Traditional Chinese Medicine Science and Technology Project of Zhejiang Province:to Investigate the Action Mechanisms of Salvia Miltiorrhiza-asarum Ointment on Osteoarthritis Based on Notch1/MMP-13 Signaling Pathway (No. 2018ZQ044)

Abstract: OBJECTIVE: This study investigated the effect of salvia miltiorrhiza-asarum ointment(SMAO) plus Chinese medical massage on knee osteoarthritis in a rat model.METHODS: Hulth's method was used to establish a Sprague-Dawley rat model of knee osteoarthritis(OA). The levels of matrix metalloproteinase-13(MMP-13), collagen-tumor necrosiⅡ, aggrecan, interleukin(IL)-1β,s factor-α(TNF-α), and IL-6 were measured by enzyme-linked immunosorbent assays. The joint space was assessed by a Perlove X-ray system. Histopathology was examined by hematoxylin-eosin and Safranin O staining. The m RNA and protein expression levels of Notch1,MMP-13, collagen-II, and aggrecan were measured by quantitative reverse transcription-polymerase chain reaction and Western blotting, respectively.RESULTS: SMAO plus Chinese medical massage significantly decreased the levels of MMP-13, IL-1β,TNF-α, and IL-6, and increased serum collagen-and aggrecan levels. Pathological injury of the kneⅡe joint was improved by SMAO treatment. m RNA and protein expression of Notch1 and MMP-13 was remarkably downregulated, but collagen-s were significantly upregulaⅡte and aggrecan leveld in cartilage tissues.CONCLUSION: SMAO combined with Chinese medical massage effectively relieves OA symptoms,which may involve inhibiting inflammation through the Notch1/MMP-13 signaling pathway.

Key words: osteoarthritis, massage, Salvia miltiorrhiza, Asarum, ointments, receptor,Notch1, matrix metalloproteinase 13