Advanced Search

Journal of Traditional Chinese Medicine ›› 2021, Vol. 41 ›› Issue (4): 581-587.DOI: 10.19852/j.cnki.jtcm.2021.04.007

Previous Articles     Next Articles

Runjing extract promotes spermatogenesis in rats with ornidazole-induced oligoasthenoteratozoospermia through extracellular signal-regulated kinase signalling, and regulating vimentin expression

YANG Kai, LI Shugen, ZHANG Tianyu, DONG Panpan, ZENG Qingqi   

  1. Department of Andrology,Zhangjiagang TCM Hospital Affiliated to Nanjing University of Chinese Medicine;Department of Urology,Suzhou Municipal Hospital;Department of Andrology,The Affiliated Hospital of Nanjing University of Chinese Medicine;Department of Andrology,Tongde Hospital of Zhejiang Province;
  • Online:2021-08-06 Published:2021-08-06
  • Supported by:
    Supported by the Suzhou Science and Technology Development Plan:Based on the Theory of Collaterals Disease and Damp Disease Explore Promoting Dampness and Dredging Collateral Methods through P38mapk Signaling Pathways Regulate Inflammation Factor of ammatory MechanismⅢRe Type Prostatitis Anti-inflsearch (No.Sysd2018174), Zhangjiagang Science and Technology Plan:Based on the Mapk Pathway Research Runjing Decoction to Oxidative Stress Injury Caused by Weak SD Rats and the Protective Effects of Disease Mechanism Research,(No.ZKS1835), Youth Science and Technology Project of Zhangjiagang Health Plan:Based on the Theory of Essence Chamber to Explore Runjing Decoction Treating Idiopathic Less Weak Sperm Disease Clinical Research and the Analysis of Security (No. ZJGQNKJ201927)

PDF

211

Abstract: OBJECTIVE: To investigate the efficacy of Runjing (RJ) extract on oligoasthenoteratozoospermia(OAT) induced by ornidazole(ORN) in rats, and to study the underlying mechanism.METHODS: Twenty-four adult male Sprague–Dawley rats were treated with normal saline(control),ORN(OAT model), ORN + 4.725 g-dose) and ORN+ 18.9 g·kg~(-1)·d~(-1) RJ extract(low·kg~(-1)·d~(-1) RJ extract(high-dose) for 4 weeks. The rats were then euthanized and sperm and testis samples were collected for analysis. Sperm count, motility and morphology were calculated by sperm suspension from cauda epididymis. Testicular histopathological changes were analyzed by hematoxylin and eosin staining and Td T mediated d UTP nick end labelling. Moreover, the expression of vimentin and extracellular signal-regulated kinase(ERK)were examined through Western blot, and the distribution of vimentin was detected via immunohistochemistry.RESULTS: ORN successfully induces seminiferous epithelium injury, cellular apoptosis, and finally OAT(P < 0.05). However, both low-dose and highdose RJ extract partially rescues the altered phenotypes(P < 0.05). Moreover, the expressions of vimentin and ERK were significantly altered in ORN testes(all P < 0.001), while RJ extract partially reversed these effects(P < 0.01 or P < 0.001).CONCLUSION: RJ extract can help maintain spermatogenesis through ERK signalling, and regulating vimentin expression.

Key words: spermatogenesis, oligospermia, vimentin, extracellular signal-regulated MAP kinases, Runjing