Scalp acupuncture Yikang therapy on Baihui (GV20), Sishencong (EX-HN1), Zhisanzhen, Niesanzhen improves neurobehavior in young rats with cerebral palsy through Notch signaling pathway

doi:10.19852/j.cnki.jtcm.20221206.002

Journal of Traditional Chinese Medicine ›› 2023, Vol. 43 ›› Issue (2): 337-342.DOI: 10.19852/j.cnki.jtcm.20221206.002

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Scalp acupuncture Yikang therapy on Baihui (GV20), Sishencong (EX-HN1), Zhisanzhen, Niesanzhen improves neurobehavior in young rats with cerebral palsy through Notch signaling pathway

XUE Jianyi¹, XU Jinyan¹, HUANG Mao², YU Wentao¹, YAN Yihui¹, YAN Yuanjie¹, YIN Zhenjin³(), LU Qian², PENG Wanying², YAN Siyang⁴

1 Department of Acupuncture and Message, Hebei University of Chinese Medicine, Shijiazhuang 050091, China
2 Department of Child Rehabilitation, the First Affiliated Hospital of Hebei University of Chinese Medicine, Shijiazhuang 050013, China
3 the second Outpatient Department, Hebei University of Chinese Medicine, Shijiazhuang 050090, China
4 School of Basic Medicine, Hebei University of Chinese Medicine, Shijiazhuang 050200, China

Received:2022-02-12 Accepted:2022-04-21 Online:2023-04-15 Published:2023-03-14
Contact: YIN Zhenjin, Department of Acupuncture and Message, Hebei University of Chinese Medicine, Shijiazhuang 050091, China. yinzhenjin@126.com. Telephone: +86-19930617582
Supported by:
Key Projects of Scientific Research in Higher Education Institutions in Hebei Province: Study on the Mechanism of Scalp Acupuncture Yikang Therapy in Cerebral Palsy Rats Based on Notch Signal Pathway(ZD2020144);Doctoral Research Project of Hebei University of Chinese Medicine: Study on the Mechanism of Scalp Acupuncture Yikang Therapy in Cerebral Palsy Rats Based on Notch Signal Pathway(BSZ2020002)

Abstract

Abstract:

OBJECTIVE: To investigate the efficacy of scalp acupuncture Yikang therapy on Baihui (GV20), Sishencong (EX-HN1), Zhisanzhen, Niesanzhen, on neurobehavior in young rats with cerebral palsy based on Notch signaling pathway.

METHODS: Thirty 7-day-old rats were randomly divided into sham, model and acupuncture, 10 rats in each group. The cerebral palsy model was established by the accepted modeling method, the acupuncture group selected "Baihui (GV20)", "Sishencong (EX-HN1)", "Zhisanzhen" and "Niesanzhen" for intervention 24 h after the model was made. The body masses were recorded before and after the treatment, respectively. After the intervention, the rats were subjected to suspension experiment, slope experiment, tactile stimulation experiment and Morris water maze experiment. After the end of the experiment, the morphological changes of hippocampal histology were observed by hematoxylin-eosin (HE) staining under light microscope, and the expression of Notch1, Notch3 and Hes5 were detected by Western blot and quantitative real-time polymerase chain reaction (PCR).

RESULTS: The changes in body mass of the rats in each group were different; in behavioral experiments, compared with the sham, the suspension time of the model was shortened, the slope experiment, tactile stimulation experiment, and escape latency time were prolonged, and the number of platform crossing was reduced in the model, compared with the model, the suspension time of the acupuncture was prolonged, the slope experiment, tactile stimulation experiment, and escape latency time were shortened, and the number of platform crossing times was increased; HE staining showed severe hippocampal damage in the model and reduced hippocampal damage in the acupuncture. Western Blot and real-time fluorescence quantitative PCR showed that the expression of Notch1, Notch3 and Hes5 were increased in the model and the expression of Notch1, Notch3, Hes5 in acupuncture were decreased.

CONCLUSIONS: Scalp acupuncture Yikang therapy may improve neurobehavior and reduce brain injury in rats with cerebral palsy by downregulating the expression of Notch1, Notch3, and Hes5.

Key words: cerebral palsy, scalp acupuncture Yikang therapy, hippocampal tissue, Notch signaling pathway

XUE Jianyi, XU Jinyan, HUANG Mao, YU Wentao, YAN Yihui, YAN Yuanjie, YIN Zhenjin, LU Qian, PENG Wanying, YAN Siyang. Scalp acupuncture Yikang therapy on Baihui (GV20), Sishencong (EX-HN1), Zhisanzhen, Niesanzhen improves neurobehavior in young rats with cerebral palsy through Notch signaling pathway[J]. Journal of Traditional Chinese Medicine, 2023, 43(2): 337-342.

Figures/Tables 5

Table 1 Body mass changes of rats in each group (g, $\bar{x}\pm s$)

Group	n	Before treatment	After treatment
Sham	10	13.7±0.3	88.9±2.2
Model	8	13.5±0.5	71.5±4.4^a
Acupuncture	10	13.3±0.6	79.0±2.4^ab

Table 2 Behavioral experiment results (s, $\bar{x}\pm s$)

Group	n	Suspension time	Reaction time	Tape tearing time	Escape latency
Sham	10	56±9	3±1	27±5	11±6
Model	8	26±5^a	10±2^a	44±7^a	65±15^a
Acupuncture	10	39±13^ab	6±1^ab	31±5^b	31±8^ab

Figure 1 HE staining results A: sham group expression of HE staining in hippocampus (×400); B: model group expression of HE staining in hippocampus (×400); C: acupuncture expression of HE staining in hippocampus (×400). Sham group: only the left common carotid artery was stripped without ligation of hypoxia and intervention. Model group: treated with left common carotid artery ligation and hypoxia without intervention. Acupuncture group: treated with left common carotid artery ligation and hypoxia and acupuncture intervention. HE: hematoxylin-eosin.

Figure 2 Western blot results A: Notch1, Notch3, Hes5 protein expression; B: Western blotting to determine the protein expression of Notch1, Notch3 and Hes5 in hippocampus. Sham group: only the left common carotid artery was stripped without ligation of hypoxia and intervention. Model group: treated with left common carotid artery ligation and hypoxia without intervention. Acupuncture group: treated with left common carotid artery ligation and hypoxia and acupuncture intervention. Compared with the sham group, aP < 0.05; compared with the model group, bP < 0.05.

Table 3 Quantitative real-time PCR results ($\bar{x}\pm s$)

Group	n	Notch1 mRNA	Notch3 mRNA	Hes5 mRNA
Sham	3	1.00±0.05	1.01±0.10	0.85±0.15
Model	3	1.19±0.10^a	1.46±0.22^a	1.21±0.27^a
Acupuncture	3	0.68±0.10^ab	0.81±0.18^b	0.51±0.19^ab

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