Molecular mechanism analysis of Miao medicine Jinwujiangu decoction (金乌健骨方) in treating osteoarthritis based on a network pharmacology approach

doi:10.19852/j.cnki.jtcm.20220519.002

Journal of Traditional Chinese Medicine ›› 2022, Vol. 42 ›› Issue (4): 576-585.DOI: 10.19852/j.cnki.jtcm.20220519.002

• Research Articles • Previous Articles Next Articles

Molecular mechanism analysis of Miao medicine Jinwujiangu decoction (金乌健骨方) in treating osteoarthritis based on a network pharmacology approach

JIANG Zong¹, YAO Xiaoling², MA Wukai¹(), TANG Fang¹()

1 Department of Rheumatology, the Second Affiliated Hospital of Guizhou University of Traditional Chinese Medicine, Guiyang 550002, China
2 Department of Traditional Chinese Medicine, Longhua District Central Hospital, Shenzhen 518110, China

Received:2021-06-11 Accepted:2021-09-23 Online:2022-08-15 Published:2022-07-12
Contact: MA Wukai,TANG Fang
About author:MA Wukai, Department of Rheumatology, the Second Affiliated Hospital of Guizhou University of Traditional Chinese Medicine, No. 83, Feishan Street, Yunyan District, Guiyang 550002, Guizhou, China. walker55@163.com, Telephone: +86-851-85556970
TANG Fang, Department of Rheumatology, the Second Affiliated Hospital of Guizhou University of Traditional Chinese Medicine, Guiyang 550002, China. tangfangfemale@163.com;
Supported by:
National Natural Science Foundation of China: Molecular Mechanism of Miao Medicine JinwuJiangu Decoction in the Treatment of Knee Osteoarthritis Based on Axin-Wnt Signal Regulation Gene;Molecular Mechanism of Miao Medicine Wuteng Paste in the Treatment of Knee Osteoarthritis Based on Wnt-Notch Signaling(81760866);Molecular Mechanism of Miao Medicine Wuteng Paste in the Treatment of Knee Osteoarthritis Based on Wnt-Notch Signaling(81760884);Research on Inheritance of Ethnic Medicine(2017YFC-1703904);Study on the Mechanism of Wenjingtongluo Formula Regulating Osteoarthritis Model Rabbits based on Axin Regulatory Factors(Qian Ke He Foundation [2016] 1011);Hundred Talent Project of Guizhou Province High-Level Innovation Talent Training Plan(Qian Ke He Platform Talent [2016] 5650);Construction of Medical Platform of Traditional Chinese Medicine (Ethnic Medicine) in the Treatment of Rheumatism in Guizhou Province(Qian Ke He Platform Talent [2018] 5707);Clinical Research Center of Rheumatology and Immunology in Guizhou Province(Qian Ke He Platform Talent [2020] 2202)

Abstract

Abstract:

OBJECTIVE: To investigate molecular mechanisms of Jinwujiangu decoction (金乌健骨方, JWJG) in treating osteoarthritis (OA) using network pharmacology analysis. METHODS: Principal active compounds of JWJG were screened out via the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP) and BATMAN-TCM, and potential targets for OA treatment were identified through Online Mendelian Inheritance in Man (OMIM) and GeneCards databases. The JWJG network diagrams of both principal chemical compound-action targets and OA treatment target-OA disease were constructed applying the Cytoscape 3.7.2 software. The diagram of protein-protein interaction network was plotted for core analysis. Meanwhile, the common targets and relevant signaling pathways involved in both networks were analyzed using the Gene Ontology function analysis and Kyoto Encyclopedia of Genes and Genomes pathway enrichment. The predicted results were ultimately verified through animal experiments. RESULTS: Effects of JWJG were indicated in acting on key targets interleukin-6, insulin, protein kinase B, glyceraldehyde-3-phosphate dehydrogenase, and mitosis-specific MRE11-RAD50-NBS1 associated protein by regulating signaling pathways of phosphoinositide 3-kinase- protein kinase B, mitogen-activated protein kinases, tumor necrosis factor, and colorectal cancer. Meanwhile, it inhibited the over-activation of signaling pathways and the release of inflammatory factors in OA treatment. Following a signaling pathway analysis utilizing network pharmacology technique, it was demonstrated that JWJG could treat OA through the Wnt/β-catenin signaling pathway verified by animal experiments. CONCLUSIONS: The present study preliminarily analyzed the pharmacological mechanism of JWJG in treating OA on a network pharmacology approach and provided a great theoretical significance for clinical application.

Key words: osteoarthritis, network pharmacology, targets, Jinwujiangu decoction

JIANG Zong, YAO Xiaoling, MA Wukai, TANG Fang. Molecular mechanism analysis of Miao medicine Jinwujiangu decoction (金乌健骨方) in treating osteoarthritis based on a network pharmacology approach[J]. Journal of Traditional Chinese Medicine, 2022, 42(4): 576-585.

Figures/Tables 6

References 25

1	Malemud CJ. Biologic basis of osteoarthritis: state of the evidence. Curr Opin Rheumatol 2015; 27: 289-94. DOI URL
2	Prieto-Alhambra D, Judge A, Javaid MK, et al. Incidence and risk factors for clinically diagnosed knee, hip and hand osteoarthritis: influences of age, gender and osteoarthritis affecting other joints. Ann Rheum Dis 2014; 73: 1659-64. DOI PMID
3	Dabholkar T, Dabholkar A, Sachiwala D. Correlation of the core stability measures with the hip strength and functional activity level in knee osteoarthritis. Int J Rehabil Res 2016; 5: 37-43.
4	Międzybłocka M, Czarnecki P. Evaluation of the effectiveness of hyaluronic acid injections in treatment of small joint osteoarthritis. Ortop Traumatol Rehabil 2018; 20: 437-43. DOI PMID
5	de l'Escalopier N, Anract P, Biau D. Surgical treatments for osteoarthritis. Ann Phys Rehabil Med 2016; 59: 227-33. DOI PMID
6	Arias-Vázquez PI, Tovilla-Zárate CA, Hernández-Díaz Y, et al. Short-term therapeutic effects of ozone in the management of pain in knee osteoarthritis: a Meta-analysis. PM R 2019; 11: 879-87. DOI URL
7	Huh SW, Shetty AA, Kim JM, et al. Autologous bone marrow mesenchymal cell induced chondrogenesis for the treatment of osteoarthritis of knee. Tissue Eng Regen Med 2016; 13: 200-9. DOI URL
8	Chen L, Lu X, Lan W, et al. Clinical study on the treatment of knee osteoarthritis with Kidney deficiency and blood stasis by Jinwu Jiangu prescription. Guiyang Zhong Yi Xue Yuan Xue Bao 2018; 40: 54-7.
9	Feng Z, Xiao Q, Lian K. Research progress of Wnt signaling pathway and osteoarthritis. Jilin Yi Xue 2017; 38: 383-6.
10	Xie W, Zheng H. Research progress of Traditional Chinese Medicine in the intervention of osteoarthritis based on Wnt/β-catenin signaling pathway. Zhong Guo Gu Zhi Shu Song Za Zhi 2018; 24: 664-70.
11	Zhou Y, Wang T, Hamilton JL, et al. Wnt/β-catenin signaling in osteoarthritis and in other forms of arthritis. Curr Rheumatol Rep 2017; 19: 53. DOI URL
12	Huang J, Huang X, Chen Z, et al. Dose conversion among different animals and healthy volunteers in pharmacological study. Zhong Guo Lin Chuang Yao Li Xue Yu Zhi Liao Xue 2004; 9: 1069-72.
13	Mankin HJ, Dorfman H, Lippiello L, et al. Biochemical and metabolic abnormalities in articular cartilage from osteo-arthritic human hips. II. Correlation of morphology with biochemical and metabolic data. J Bone Joint Surg Am 1971; 53: 523-37. PMID
14	Yang M, Jiang L, Wang Q, et al. Traditional Chinese Medicine for knee osteoarthritis: an overview of systematic review. PLoS One 2017; 12: e0189884.
15	Xu YK, Zhu FG, Feng EY, et al. External therapies of traditional Chinese medicine combined with sodium hyaluronate injected in articular cavity therapy on knee osteoarthritis: Meta-analysis. Zhong Guo Zhong Yao Za Zhi 2018; 43: 1934-9.
16	Lo PC, Lin FC, Tsai YC, et al. Traditional Chinese Medicine therapy reduces the risk of total knee replacement in patients with knee osteoarthritis. Medicine (Baltimore) 2019; 98: e15964. DOI URL
17	Gu P, Zhu L, Liu Y, et al. Protective effects of paeoniflorin on TNBS-induced ulcerative colitis through inhibiting NF-kappa B pathway and apoptosis in mice. Int Immunopharmacol 2017; 50: 152-60. DOI URL
18	Arai E, Sakamoto H, Ichikawa H, et al. Multilayer-omics analysis of renal cell carcinoma, including the whole exome, methylome and transcriptome. Int J Cancer 2014; 135: 1330-42. DOI PMID
19	Song X, Cai W, Li L. Axin PPI networks: new interacting proteins and new targets? Curr Top Med Chem 2016; 16: 3678-90. DOI URL
20	Hwang HS, Kim HA. Chondrocyte apoptosis in the pathogenesis of osteoarthritis. Int J Mol Sci 2015; 16: 26035-54. DOI PMID
21	Ma B, Landman EBM, Miclea RL, et al. WNT signaling and cartilage: of mice and men. Calcif Tissue Int 2013; 92: 399-411. DOI URL
22	Schmitz Y, Rateitschak K, Wolkenhauer O. Analysing the impact of nucleo-cytoplasmic shuttling of β-catenin and its antagonists APC, Axin and GSK3 on Wnt/β-catenin signalling. Cell Signal 2013; 25: 2210-21.
23	Zhang L, Ma S, Su H, et al. Isoliquiritigenin inhibits IL-1beta-induced production of matrix metalloproteinase in articular chondrocytes. Mol Ther Methods Clin Dev 2018; 9: 153-9. DOI URL
24	Gillis WQ, Kirmizitas A, Iwasaki Y, et al Gtpbp2 is a positive regulator of Wnt signaling and maintains low levels of the Wnt negative regulator Axin. Cell Commun Signal 2016; 14: 15. DOI URL
25	Li J, Zhou XD, Yang KH, et al. Hinokitiol reduces matrix metalloproteinase expression by inhibiting Wnt/β-Catenin signaling in vitro and in vivo. Int Immunopharmacol 2014; 23: 85-91. DOI URL

Chinese Herb	Mol ID	Molecule Name	OB	DL
Baishao (Radix Paeoniae Alba)	MOL001910	11alpha,12alpha-epoxy-3beta-23-dihydroxy- 30-norolean-20-en-28,12beta-olide	64.77	0.38
	MOL001918	paeoniflorgenone	87.59	0.37
	MOL001919	(3S,5R,8R,9R,10S,14S)-3,17-dihydroxy-4,4,8,10,14- pentamethyl-2,3,5,6,7,9-hexahydro-1H- cyclopenta[a]phenanthrene-15,16-dione	43.56	0.53
	MOL001921	Lactiflorin	49.12	0.8
	MOL001924	paeoniflorin	53.87	0.79
	MOL001925	paeoniflorin_qt	68.18	0.4
	MOL001928	albiflorin_qt	66.64	0.33
	MOL001930	benzoyl paeoniflorin	31.27	0.75
	MOL000211	Mairin	55.38	0.78
	MOL000358	beta-sitosterol	36.91	0.75
	MOL000359	sitosterol	36.91	0.75
	MOL000422	kaempferol	41.88	0.24
	MOL000492	-catechin	54.83	0.24
Jianghuang (Rhizoma Curcumae Longae)	MOL000449	Stigmasterol	43.83	0.76
	MOL000493	campesterol	37.58	0.71
	MOL000953	CLR	37.87	0.68
Qiannianjian (Rhizoma Homalomenae)	MOL000358	beta-sitosterol	36.91	0.75
	MOL000686	acetylbullatantriol	40.21	0.18
	MOL000691	maristeminol	30.64	0.38
Qingfenteng (Caulis Sinomenii)	MOL000358	beta-sitosterol	36.91	0.75
	MOL000621	16-epi-Isositsirikine	49.52	0.59
	MOL000622	Magnograndiolide	63.71	0.19
	MOL000623	Michelenolide	47.54	0.25
	MOL000625	Sinomenine	46.09	0.53
	MOL000627	Stepholidine	33.11	0.54
Sanqi (Radix Notoginseng)	MOL001494	Mandenol	42	0.19
	MOL001792	DFV	32.76	0.18
	MOL002879	Diop	43.59	0.39
	MOL000358	beta-sitosterol	36.91	0.75
	MOL000449	Stigmasterol	43.83	0.76
	MOL005344	ginsenoside rh2	36.32	0.56
	MOL007475	ginsenoside f2	36.43	0.25
	MOL000098	quercetin	46.43	0.28

Chinese Herb	Mol ID	Molecule Name	OB	DL
Baishao (Radix Paeoniae Alba)	MOL001910	11alpha,12alpha-epoxy-3beta-23-dihydroxy- 30-norolean-20-en-28,12beta-olide	64.77	0.38
	MOL001918	paeoniflorgenone	87.59	0.37
	MOL001919	(3S,5R,8R,9R,10S,14S)-3,17-dihydroxy-4,4,8,10,14- pentamethyl-2,3,5,6,7,9-hexahydro-1H- cyclopenta[a]phenanthrene-15,16-dione	43.56	0.53
	MOL001921	Lactiflorin	49.12	0.8
	MOL001924	paeoniflorin	53.87	0.79
	MOL001925	paeoniflorin_qt	68.18	0.4
	MOL001928	albiflorin_qt	66.64	0.33
	MOL001930	benzoyl paeoniflorin	31.27	0.75
	MOL000211	Mairin	55.38	0.78
	MOL000358	beta-sitosterol	36.91	0.75
	MOL000359	sitosterol	36.91	0.75
	MOL000422	kaempferol	41.88	0.24
	MOL000492	-catechin	54.83	0.24
Jianghuang (Rhizoma Curcumae Longae)	MOL000449	Stigmasterol	43.83	0.76
	MOL000493	campesterol	37.58	0.71
	MOL000953	CLR	37.87	0.68
Qiannianjian (Rhizoma Homalomenae)	MOL000358	beta-sitosterol	36.91	0.75
	MOL000686	acetylbullatantriol	40.21	0.18
	MOL000691	maristeminol	30.64	0.38
Qingfenteng (Caulis Sinomenii)	MOL000358	beta-sitosterol	36.91	0.75
	MOL000621	16-epi-Isositsirikine	49.52	0.59
	MOL000622	Magnograndiolide	63.71	0.19
	MOL000623	Michelenolide	47.54	0.25
	MOL000625	Sinomenine	46.09	0.53
	MOL000627	Stepholidine	33.11	0.54
Sanqi (Radix Notoginseng)	MOL001494	Mandenol	42	0.19
	MOL001792	DFV	32.76	0.18
	MOL002879	Diop	43.59	0.39
	MOL000358	beta-sitosterol	36.91	0.75
	MOL000449	Stigmasterol	43.83	0.76
	MOL005344	ginsenoside rh2	36.32	0.56
	MOL007475	ginsenoside f2	36.43	0.25
	MOL000098	quercetin	46.43	0.28

Chinese herb	Mol ID	Molecule Name	Mol ID	Molecule Name
Jinmaogouji (Cibotium Barometz)	MOL001973	β-Sitosterol	MOL001988	Stearic acid
	MOL001974	Hexadecanoic acid	MOL001989	Diethyl phthalate
	MOL001975	5-Hydroxymethyl furaldehyde	MOL001990	Tetradecanoic acid
	MOL001976	Cirsiumaldehyde	MOL001991	Linoleic acid ethyl ester
	MOL001977	Daucosterol	MOL001992	Methyl palmitate
	MOL001978	3-hydroxyl-γ-pyrone	MOL001993	Ethyl Stearate
	MOL001979	γ-Terpinen	MOL001993	p-Hydroxyacetanilide
	MOL001980	Piperitone	MOL001995	Protocatechuic aldehyde
	MOL001981	Cadina-1(10)4-diene	MOL001996	Vanillin
	MOL001982	7,10,13-Hexadecatrienoic acid methyl ester	MOL001998	(24R)-stigmnst-4-ene-3-one
	MOL001983	3-Terpineol	MOL001999	24-methylenecyeloartanol
	MOL001984	-5,11,14,17-eicosatetraenoate methyl	MOL002000	(3R)-des-O-methyl-lasiodiplodin
	MOL001989	Dibutyl phthalate	MOL002001	onitin
	MOL001985	-9-Octadecenoic acid	MOL002003	onitin·2'-O-β-D-glucopyranosid
	MOL001986	Palmitic acid	MOL001997	kaempferide
	MOL001987	Linolenic acid methyl ester	MOL002002	altemariol
	MOL001984	(Z,Z,Z)-9,12,15-Octadecatrien-1-o1
Heiguteng (Periploca Forrestii Schltr)	MOL001374	△5-pregnene-3β,17α,20α-triol	MOL001061	3-O-caffeoylquinic acid methyl ester
	MOL000016	27-hydroxyl-α-amyrin	MOL001233	Syringic cacid
	MOL000031	Ursolic acid	MOL001245	vanillic acid
	MOL000033	β-Sitosterol	MOL000001	α-Amyrin
	MOL000047	daucosterol	MOL001402	Chrysophanol
	MOL000061	Emodin	MOL001608	TanshinoneⅡA
	MOL000225	Physcion	MOL001685	Soy isoflavone
	MOL000241	Phenacetin	MOL001888	Liquiritigenin
	MOL000274	Hexadecanoic acid	MOL001916	Linalool
	MOL000404	3β-hydroxy-oleana-ll, 13(18)-dien-28-oic acid	MOL001075	1,3-di-O一caffeoylquinic acid
	MOL000407	14-ursen-3-ol-1-one	MOL001816	Formononetin
	MOL000433	kaempferol	MOL001089	Protocatechuic aldehyde
	MOL000481	quercetin	MOL001943	Anethole?
	MOL000536	scopoletin	MOL000936	n-Heptadecane
	MOL000522	emodin-8-O-β-D-glucopyranoside	MOL000508	physcion-8-O-β-D-glucopyranoside
	MOL000666	E-p-hydroxy-cinnamic acid	MOL000867	caffeicacid
	MOL000498	kaempferol-3-O-β-D-galactopyranoside
Wushaoshe (Zaocys)	MOL000017	ihydroferulic acid	MOL000145	Thymine
	MOL000131	β-sitosterol	MOL000283	4-hydroxybenzaIdehyde
	MOL000001	utylisobutyl phthalate

Chinese herb	Mol ID	Molecule Name	Mol ID	Molecule Name
Jinmaogouji (Cibotium Barometz)	MOL001973	β-Sitosterol	MOL001988	Stearic acid
	MOL001974	Hexadecanoic acid	MOL001989	Diethyl phthalate
	MOL001975	5-Hydroxymethyl furaldehyde	MOL001990	Tetradecanoic acid
	MOL001976	Cirsiumaldehyde	MOL001991	Linoleic acid ethyl ester
	MOL001977	Daucosterol	MOL001992	Methyl palmitate
	MOL001978	3-hydroxyl-γ-pyrone	MOL001993	Ethyl Stearate
	MOL001979	γ-Terpinen	MOL001993	p-Hydroxyacetanilide
	MOL001980	Piperitone	MOL001995	Protocatechuic aldehyde
	MOL001981	Cadina-1(10)4-diene	MOL001996	Vanillin
	MOL001982	7,10,13-Hexadecatrienoic acid methyl ester	MOL001998	(24R)-stigmnst-4-ene-3-one
	MOL001983	3-Terpineol	MOL001999	24-methylenecyeloartanol
	MOL001984	-5,11,14,17-eicosatetraenoate methyl	MOL002000	(3R)-des-O-methyl-lasiodiplodin
	MOL001989	Dibutyl phthalate	MOL002001	onitin
	MOL001985	-9-Octadecenoic acid	MOL002003	onitin·2'-O-β-D-glucopyranosid
	MOL001986	Palmitic acid	MOL001997	kaempferide
	MOL001987	Linolenic acid methyl ester	MOL002002	altemariol
	MOL001984	(Z,Z,Z)-9,12,15-Octadecatrien-1-o1
Heiguteng (Periploca Forrestii Schltr)	MOL001374	△5-pregnene-3β,17α,20α-triol	MOL001061	3-O-caffeoylquinic acid methyl ester
	MOL000016	27-hydroxyl-α-amyrin	MOL001233	Syringic cacid
	MOL000031	Ursolic acid	MOL001245	vanillic acid
	MOL000033	β-Sitosterol	MOL000001	α-Amyrin
	MOL000047	daucosterol	MOL001402	Chrysophanol
	MOL000061	Emodin	MOL001608	TanshinoneⅡA
	MOL000225	Physcion	MOL001685	Soy isoflavone
	MOL000241	Phenacetin	MOL001888	Liquiritigenin
	MOL000274	Hexadecanoic acid	MOL001916	Linalool
	MOL000404	3β-hydroxy-oleana-ll, 13(18)-dien-28-oic acid	MOL001075	1,3-di-O一caffeoylquinic acid
	MOL000407	14-ursen-3-ol-1-one	MOL001816	Formononetin
	MOL000433	kaempferol	MOL001089	Protocatechuic aldehyde
	MOL000481	quercetin	MOL001943	Anethole?
	MOL000536	scopoletin	MOL000936	n-Heptadecane
	MOL000522	emodin-8-O-β-D-glucopyranoside	MOL000508	physcion-8-O-β-D-glucopyranoside
	MOL000666	E-p-hydroxy-cinnamic acid	MOL000867	caffeicacid
	MOL000498	kaempferol-3-O-β-D-galactopyranoside
Wushaoshe (Zaocys)	MOL000017	ihydroferulic acid	MOL000145	Thymine
	MOL000131	β-sitosterol	MOL000283	4-hydroxybenzaIdehyde
	MOL000001	utylisobutyl phthalate

No.	KEGG Signaling pathways	Gene counts	Rate (%)
1	Kaposi sarcoma-associated herpesvirus infection	39	20.74
2	Hepatitis B	37	19.68
3	Human cytomegalovirus infection	36	19.15
4	AGE-RAGE signaling pathway in diabetic complications	33	17.55
5	PI3K-Akt signaling pathway	33	17.55
6	Fluid shear stress and atherosclerosis	32	17.02
7	MAPK signaling pathway	31	16.49
8	Human T-cell leukemia virus 1 infection	30	15.96
9	Measles	29	15.43
10	Epstein-Barr virus infection	29	15.43
11	TNF signaling pathway	28	14.89
12	Tuberculosis	28	14.89
13	Prostate cancer	27	14.36
14	Toll-like receptor signaling pathway	27	14.36
15	Influenza A	27	14.36
16	IL-17 signaling pathway	26	13.83
17	Chagas disease (American trypanosomiasis)	26	13.83
18	Yersinia infection	26	13.83
19	Hepatitis C	26	13.83
20	Proteoglycans in cancer	26	13.83
109	Wnt signaling pathway	9	4.79
110	Cytosolic DNA-sensing pathway	9	4.79

Home

Online First

Author Center

Reviewer Center

Issues

Announcement

About Us

Molecular mechanism analysis of Miao medicine Jinwujiangu decoction (金乌健骨方) in treating osteoarthritis based on a network pharmacology approach

RichHTML

PDF

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 6

References 25

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	ZHI Guoguo, SHAO Bingjie, ZHENG Tianyan, JI Shaoxiu, LI Jingwei, DANG Yanni, LIU Feng, WANG Dong. Efficacy of Ganshuang granules (肝爽颗粒) on non-alcoholic fatty liver and underlying mechanism: a network pharmacology and experimental verification [J]. Journal of Traditional Chinese Medicine, 2024, 44(1): 122-130.
[2]	YANG Ye, CHEN Xiaoyang, YAO Junkai, HU Yueyao, WANG Wei. Efficacy of Danlou tablet (丹蒌片) on myocardial ischemia/ reperfusion injury assessed by network pharmacology and experimental verification [J]. Journal of Traditional Chinese Medicine, 2024, 44(1): 131-144.
[3]	ZHANG Qi, CHEN Dexuan, ZHU Guixiang, ZHANG Shihu, FENG Xiao, MA Chaoqun, ZHANG Yi. Efficacy of Tounongsan decoction (透脓散方) on pyogenic liver abscess: network pharmacology and clinical trial validation [J]. Journal of Traditional Chinese Medicine, 2024, 44(1): 145-155.
[4]	REN Hui, ZHAO Lintao, GAO Kai, YANG Yuanyuan, CUI Xiaomin, HU Jing, CHEN Zhiyong, LI Ye. Deciphering the chemical profile and pharmacological mechanism of Jinlingzi powder (金铃子散) against bile reflux gastritis using ultra-high performance liquid chromatography coupled with Q exactive focus mass spectrometry, network pharmacology, and molecular docking [J]. Journal of Traditional Chinese Medicine, 2023, 43(6): 1209-1218.
[5]	YANG Xirui, ZHAO Hui, SHAN Muhammad, DONG Feixue, ZHANG Dandan, WANG Jixue, YUAN Xingxing. Efficacy of bioactive compounds of Chaihu (Radix Bupleuri Chinensis) on glaucomatous optic atrophy through interleukin-6/hypoxia inducible factor-1α signal pathway [J]. Journal of Traditional Chinese Medicine, 2023, 43(6): 1219-1226.
[6]	HAN Huagang, LI Ziqiang, OUYANG Jingfeng, WANG Tianquan, DONG Lingyan, CAO Junling. Mechanism of Lingbao Huxin Dan (灵宝护心丹) in the treatment of bradyarrhythmia complicated with coronary heart disease: a network pharmacology analysis [J]. Journal of Traditional Chinese Medicine, 2023, 43(5): 1001-1009.
[7]	DING Luobin, WANG Huajun, LI Yao, LI Jia, LI Ling, GAO Yangping, GUAN Jian, GENG Weiqiang. Electroacupuncture stimulating Neixiyan (EX-LE5) and Dubi (ST35) alleviates osteoarthritis in rats induced by anterior cruciate ligament transaction via affecting DNA methylation regulated transcription of miR-146a and miR-140-5p [J]. Journal of Traditional Chinese Medicine, 2023, 43(5): 983-990.
[8]	Chen Xilin, GUO Yan, LU Juan, QIN Luxue, HU Tingyao, ZENG Xin, WANG Xinyue, ZHANG Anran, ZHUANG Yuxin, ZHONG Honggang, GUO Changqing. Acupotomy ameliorates subchondral bone absorption and mechanical properties in rabbits with knee osteoarthritis by regulating bone morphogenetic protein 2-Smad1 pathway [J]. Journal of Traditional Chinese Medicine, 2023, 43(4): 734-743.
[9]	PANG Fengtao, LI Kesong, ZHANG Yi, TANG Xiaopo, ZHOU Xinyao. Efficacy of Lushi Runzao decoction (路氏润燥汤) on ameliorating Sjogren's syndrome: a network pharmacology and experimental verification-based study [J]. Journal of Traditional Chinese Medicine, 2023, 43(4): 751-759.
[10]	JIA Lihua, KUANG Haodan, XU Yuan. Efficacy of Buzhong Yiqi decoction (补中益气汤) on benign prostatic hyperplasia and its possible mechanism [J]. Journal of Traditional Chinese Medicine, 2023, 43(3): 533-541.
[11]	JIA Lihong, TIE Defu, FAN Zhaohui, CHEN Dan, CHEN Qizhu, CHEN Jun, BO Huaben. Mechanism underlying Fanmugua (Fructus Caricae) leaf multicomponent synergistic therapy for anemia: data mining based on hematopoietic network [J]. Journal of Traditional Chinese Medicine, 2023, 43(3): 542-551.
[12]	GUO Zhuang, WANG Junwen, LI Zhonglong, CHEN Zhongjie, CHEN Li, YAN Shiyan, LU Hongrong, LI Zhigeng, LI Guanying. Protocol to establish auxiliary diagnostic model for knee osteoarthritis functional testing equipment [J]. Journal of Traditional Chinese Medicine, 2023, 43(2): 379-385.
[13]	LI Yue, WEN Shuting, ZHAO Runyuan, FAN Dongmei, ZHAO Dike, LIU Fengbin, MI Hong. Efficacy of active ingredients in Qingdai (Indigo Naturalis) on ulcerative colitis: a network pharmacology-based evaluation [J]. Journal of Traditional Chinese Medicine, 2023, 43(1): 124-133.
[14]	ZHOU Kaixuan, ZHANG Dong, BAO Huiwei, LI Lijing. Network pharmacology and molecular docking study on the effect of Kaempferol in treatment of metabolic associated fatty liver disease [J]. Journal of Traditional Chinese Medicine, 2022, 42(5): 788-794.
[15]	QIN Luxue, GUO Changqing, ZHAO Ruili, WANG Tong, WANG Junmei, GUO Yan, ZHANG Wei, HU Tingyao, CHEN Xilin, ZHANG Qian, ZHANG Dian, XU Yue. Acupotomy inhibits aberrant formation of subchondral bone through regulating osteoprotegerin/receptor activator of nuclear factor-κB ligand pathway in rabbits with knee osteoarthritis induced by modified Videman method [J]. Journal of Traditional Chinese Medicine, 2022, 42(3): 389-399.